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3xflag tagged bg4  (Addgene inc)


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    Addgene inc 3xflag tagged bg4
    Analysis of G4 focus formation in FANCJ -KO HeLa cells. ( A ) Immunofluorescence showing <t>BG4</t> foci in the indicated cell lines. HeLa cells were treated for 24 h with PDS (10 μM). Scale bar, 5 μm. ( B ) Fold increase of the BG4 CTF, detected per nucleus in the indicated cell lines and conditions. The chart reports medians (lines), mean (cross), 50% (box), and 90% (whiskers) of the datasets and outliers (circles). A total of 100 cells were analysed per condition in three biological independent experiments. Statistical analyses were performed using a non-parametrical Mann–Whitney U -test. Two-tailed P -values were calculated (**** P < .0001).
    3xflag Tagged Bg4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3xflag+tagged+bg4/pET-deSpCas9-VP64-6xHis+(Plasmid+%2392117)/pmc11806260-48-1-10
    Average 86 stars, based on 1 article reviews
    3xflag tagged bg4 - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "Effects of hydrazone-based G-quadruplex ligands on FANCJ/BRIP1 -depleted cancer cells and a Caenorhabditis elegans dog-1 −/− strain"

    Article Title: Effects of hydrazone-based G-quadruplex ligands on FANCJ/BRIP1 -depleted cancer cells and a Caenorhabditis elegans dog-1 −/− strain

    Journal: NAR Cancer

    doi: 10.1093/narcan/zcaf004

    Analysis of G4 focus formation in FANCJ -KO HeLa cells. ( A ) Immunofluorescence showing BG4 foci in the indicated cell lines. HeLa cells were treated for 24 h with PDS (10 μM). Scale bar, 5 μm. ( B ) Fold increase of the BG4 CTF, detected per nucleus in the indicated cell lines and conditions. The chart reports medians (lines), mean (cross), 50% (box), and 90% (whiskers) of the datasets and outliers (circles). A total of 100 cells were analysed per condition in three biological independent experiments. Statistical analyses were performed using a non-parametrical Mann–Whitney U -test. Two-tailed P -values were calculated (**** P < .0001).
    Figure Legend Snippet: Analysis of G4 focus formation in FANCJ -KO HeLa cells. ( A ) Immunofluorescence showing BG4 foci in the indicated cell lines. HeLa cells were treated for 24 h with PDS (10 μM). Scale bar, 5 μm. ( B ) Fold increase of the BG4 CTF, detected per nucleus in the indicated cell lines and conditions. The chart reports medians (lines), mean (cross), 50% (box), and 90% (whiskers) of the datasets and outliers (circles). A total of 100 cells were analysed per condition in three biological independent experiments. Statistical analyses were performed using a non-parametrical Mann–Whitney U -test. Two-tailed P -values were calculated (**** P < .0001).

    Techniques Used: Immunofluorescence, MANN-WHITNEY, Two Tailed Test

    Quantitative analysis of G4 stabilization in FANCJ -KO cells by different G4 binders. ( A ) Immunofluorescence representative images showing BG4 foci in the indicated cell lines after treatment with PDS (500 nM), FIM (500 nM), and FIM-15 (250 nM) for 24 h. Scale bar, 5 μm. ( B ) Fold increase of the BG4 CTF, detected per nucleus in the indicated cell lines and conditions. The chart reports medians (lines), mean (cross), 50% (box), and 90% (whiskers) of the datasets and outliers (circles). A total of 100 cells were analysed per condition in three biological independent experiments. Statistical analyses were performed using a non-parametrical Mann–Whitney U -test. Calculated P -values were indicated as * P < .05; **** P < .0001.
    Figure Legend Snippet: Quantitative analysis of G4 stabilization in FANCJ -KO cells by different G4 binders. ( A ) Immunofluorescence representative images showing BG4 foci in the indicated cell lines after treatment with PDS (500 nM), FIM (500 nM), and FIM-15 (250 nM) for 24 h. Scale bar, 5 μm. ( B ) Fold increase of the BG4 CTF, detected per nucleus in the indicated cell lines and conditions. The chart reports medians (lines), mean (cross), 50% (box), and 90% (whiskers) of the datasets and outliers (circles). A total of 100 cells were analysed per condition in three biological independent experiments. Statistical analyses were performed using a non-parametrical Mann–Whitney U -test. Calculated P -values were indicated as * P < .05; **** P < .0001.

    Techniques Used: Immunofluorescence, MANN-WHITNEY

    Detection of G4 foci in the gonadal nuclei of C. elegans dog-1 − / − . The indicated worm strains were subjected to a 24-h treatment with FIM-15 (20 μM). Nuclei were co-stained with the BG4 antibody and DAPI. Scale bar, 20 μm. Insets, indicated by boxes, show sections of the main images corresponding to the mitotic zone and pachytene stage nuclei. Scale bar, 5 μm.
    Figure Legend Snippet: Detection of G4 foci in the gonadal nuclei of C. elegans dog-1 − / − . The indicated worm strains were subjected to a 24-h treatment with FIM-15 (20 μM). Nuclei were co-stained with the BG4 antibody and DAPI. Scale bar, 20 μm. Insets, indicated by boxes, show sections of the main images corresponding to the mitotic zone and pachytene stage nuclei. Scale bar, 5 μm.

    Techniques Used: Staining

    Related Articles

    Recombinant:

    Article Title: Effects of hydrazone-based G-quadruplex ligands on FANCJ/BRIP1 -depleted cancer cells and a Caenorhabditis elegans dog-1 −/− strain
    Article Snippet: .. Recombinant 6xHis/3xFLAG-tagged BG4 was produced in Escherichia coli using pSANG10-3F-BG4 (Addgene; #55756) as follows: BL21(DE3) competent cells were transformed with pSANG10-3F plasmid expressing BG4 scFv anti-G4 antibody. .. The transformed cells were grown in 3 L of 2× TY medium [1.6% (w:v) bacto tryptone, 1% (w:v) bacto yeast extract, 0.5% (w:v) NaCl] containing 1% (w:v) glucose and 50 μg/mL kanamycin, for 24 h at 18°C.

    Produced:

    Article Title: Effects of hydrazone-based G-quadruplex ligands on FANCJ/BRIP1 -depleted cancer cells and a Caenorhabditis elegans dog-1 −/− strain
    Article Snippet: .. Recombinant 6xHis/3xFLAG-tagged BG4 was produced in Escherichia coli using pSANG10-3F-BG4 (Addgene; #55756) as follows: BL21(DE3) competent cells were transformed with pSANG10-3F plasmid expressing BG4 scFv anti-G4 antibody. .. The transformed cells were grown in 3 L of 2× TY medium [1.6% (w:v) bacto tryptone, 1% (w:v) bacto yeast extract, 0.5% (w:v) NaCl] containing 1% (w:v) glucose and 50 μg/mL kanamycin, for 24 h at 18°C.

    Transformation Assay:

    Article Title: Effects of hydrazone-based G-quadruplex ligands on FANCJ/BRIP1 -depleted cancer cells and a Caenorhabditis elegans dog-1 −/− strain
    Article Snippet: .. Recombinant 6xHis/3xFLAG-tagged BG4 was produced in Escherichia coli using pSANG10-3F-BG4 (Addgene; #55756) as follows: BL21(DE3) competent cells were transformed with pSANG10-3F plasmid expressing BG4 scFv anti-G4 antibody. .. The transformed cells were grown in 3 L of 2× TY medium [1.6% (w:v) bacto tryptone, 1% (w:v) bacto yeast extract, 0.5% (w:v) NaCl] containing 1% (w:v) glucose and 50 μg/mL kanamycin, for 24 h at 18°C.

    Plasmid Preparation:

    Article Title: Effects of hydrazone-based G-quadruplex ligands on FANCJ/BRIP1 -depleted cancer cells and a Caenorhabditis elegans dog-1 −/− strain
    Article Snippet: .. Recombinant 6xHis/3xFLAG-tagged BG4 was produced in Escherichia coli using pSANG10-3F-BG4 (Addgene; #55756) as follows: BL21(DE3) competent cells were transformed with pSANG10-3F plasmid expressing BG4 scFv anti-G4 antibody. .. The transformed cells were grown in 3 L of 2× TY medium [1.6% (w:v) bacto tryptone, 1% (w:v) bacto yeast extract, 0.5% (w:v) NaCl] containing 1% (w:v) glucose and 50 μg/mL kanamycin, for 24 h at 18°C.

    Expressing:

    Article Title: Effects of hydrazone-based G-quadruplex ligands on FANCJ/BRIP1 -depleted cancer cells and a Caenorhabditis elegans dog-1 −/− strain
    Article Snippet: .. Recombinant 6xHis/3xFLAG-tagged BG4 was produced in Escherichia coli using pSANG10-3F-BG4 (Addgene; #55756) as follows: BL21(DE3) competent cells were transformed with pSANG10-3F plasmid expressing BG4 scFv anti-G4 antibody. .. The transformed cells were grown in 3 L of 2× TY medium [1.6% (w:v) bacto tryptone, 1% (w:v) bacto yeast extract, 0.5% (w:v) NaCl] containing 1% (w:v) glucose and 50 μg/mL kanamycin, for 24 h at 18°C.



    Similar Products

    3xflag tagged bg4  (Addgene inc)
    86
    Addgene inc 3xflag tagged bg4
    Analysis of G4 focus formation in FANCJ -KO HeLa cells. ( A ) Immunofluorescence showing <t>BG4</t> foci in the indicated cell lines. HeLa cells were treated for 24 h with PDS (10 μM). Scale bar, 5 μm. ( B ) Fold increase of the BG4 CTF, detected per nucleus in the indicated cell lines and conditions. The chart reports medians (lines), mean (cross), 50% (box), and 90% (whiskers) of the datasets and outliers (circles). A total of 100 cells were analysed per condition in three biological independent experiments. Statistical analyses were performed using a non-parametrical Mann–Whitney U -test. Two-tailed P -values were calculated (**** P < .0001).
    3xflag Tagged Bg4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3xflag+tagged+bg4/pET-deSpCas9-VP64-6xHis+(Plasmid+%2392117)/pmc11806260-48-1-10
    Average 86 stars, based on 1 article reviews
    3xflag tagged bg4 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Analysis of G4 focus formation in FANCJ -KO HeLa cells. ( A ) Immunofluorescence showing BG4 foci in the indicated cell lines. HeLa cells were treated for 24 h with PDS (10 μM). Scale bar, 5 μm. ( B ) Fold increase of the BG4 CTF, detected per nucleus in the indicated cell lines and conditions. The chart reports medians (lines), mean (cross), 50% (box), and 90% (whiskers) of the datasets and outliers (circles). A total of 100 cells were analysed per condition in three biological independent experiments. Statistical analyses were performed using a non-parametrical Mann–Whitney U -test. Two-tailed P -values were calculated (**** P < .0001).

    Journal: NAR Cancer

    Article Title: Effects of hydrazone-based G-quadruplex ligands on FANCJ/BRIP1 -depleted cancer cells and a Caenorhabditis elegans dog-1 −/− strain

    doi: 10.1093/narcan/zcaf004

    Figure Lengend Snippet: Analysis of G4 focus formation in FANCJ -KO HeLa cells. ( A ) Immunofluorescence showing BG4 foci in the indicated cell lines. HeLa cells were treated for 24 h with PDS (10 μM). Scale bar, 5 μm. ( B ) Fold increase of the BG4 CTF, detected per nucleus in the indicated cell lines and conditions. The chart reports medians (lines), mean (cross), 50% (box), and 90% (whiskers) of the datasets and outliers (circles). A total of 100 cells were analysed per condition in three biological independent experiments. Statistical analyses were performed using a non-parametrical Mann–Whitney U -test. Two-tailed P -values were calculated (**** P < .0001).

    Article Snippet: Recombinant 6xHis/3xFLAG-tagged BG4 was produced in Escherichia coli using pSANG10-3F-BG4 (Addgene; #55756) as follows: BL21(DE3) competent cells were transformed with pSANG10-3F plasmid expressing BG4 scFv anti-G4 antibody.

    Techniques: Immunofluorescence, MANN-WHITNEY, Two Tailed Test

    Quantitative analysis of G4 stabilization in FANCJ -KO cells by different G4 binders. ( A ) Immunofluorescence representative images showing BG4 foci in the indicated cell lines after treatment with PDS (500 nM), FIM (500 nM), and FIM-15 (250 nM) for 24 h. Scale bar, 5 μm. ( B ) Fold increase of the BG4 CTF, detected per nucleus in the indicated cell lines and conditions. The chart reports medians (lines), mean (cross), 50% (box), and 90% (whiskers) of the datasets and outliers (circles). A total of 100 cells were analysed per condition in three biological independent experiments. Statistical analyses were performed using a non-parametrical Mann–Whitney U -test. Calculated P -values were indicated as * P < .05; **** P < .0001.

    Journal: NAR Cancer

    Article Title: Effects of hydrazone-based G-quadruplex ligands on FANCJ/BRIP1 -depleted cancer cells and a Caenorhabditis elegans dog-1 −/− strain

    doi: 10.1093/narcan/zcaf004

    Figure Lengend Snippet: Quantitative analysis of G4 stabilization in FANCJ -KO cells by different G4 binders. ( A ) Immunofluorescence representative images showing BG4 foci in the indicated cell lines after treatment with PDS (500 nM), FIM (500 nM), and FIM-15 (250 nM) for 24 h. Scale bar, 5 μm. ( B ) Fold increase of the BG4 CTF, detected per nucleus in the indicated cell lines and conditions. The chart reports medians (lines), mean (cross), 50% (box), and 90% (whiskers) of the datasets and outliers (circles). A total of 100 cells were analysed per condition in three biological independent experiments. Statistical analyses were performed using a non-parametrical Mann–Whitney U -test. Calculated P -values were indicated as * P < .05; **** P < .0001.

    Article Snippet: Recombinant 6xHis/3xFLAG-tagged BG4 was produced in Escherichia coli using pSANG10-3F-BG4 (Addgene; #55756) as follows: BL21(DE3) competent cells were transformed with pSANG10-3F plasmid expressing BG4 scFv anti-G4 antibody.

    Techniques: Immunofluorescence, MANN-WHITNEY

    Detection of G4 foci in the gonadal nuclei of C. elegans dog-1 − / − . The indicated worm strains were subjected to a 24-h treatment with FIM-15 (20 μM). Nuclei were co-stained with the BG4 antibody and DAPI. Scale bar, 20 μm. Insets, indicated by boxes, show sections of the main images corresponding to the mitotic zone and pachytene stage nuclei. Scale bar, 5 μm.

    Journal: NAR Cancer

    Article Title: Effects of hydrazone-based G-quadruplex ligands on FANCJ/BRIP1 -depleted cancer cells and a Caenorhabditis elegans dog-1 −/− strain

    doi: 10.1093/narcan/zcaf004

    Figure Lengend Snippet: Detection of G4 foci in the gonadal nuclei of C. elegans dog-1 − / − . The indicated worm strains were subjected to a 24-h treatment with FIM-15 (20 μM). Nuclei were co-stained with the BG4 antibody and DAPI. Scale bar, 20 μm. Insets, indicated by boxes, show sections of the main images corresponding to the mitotic zone and pachytene stage nuclei. Scale bar, 5 μm.

    Article Snippet: Recombinant 6xHis/3xFLAG-tagged BG4 was produced in Escherichia coli using pSANG10-3F-BG4 (Addgene; #55756) as follows: BL21(DE3) competent cells were transformed with pSANG10-3F plasmid expressing BG4 scFv anti-G4 antibody.

    Techniques: Staining